Abstract
To explore the expression of astrocyte elevated gene-1 (AEG-1) in cervical cancer and analyze its correlation with microvascular density (MVD), nuclear factor kappaB (NF-kB p65) and vascular endothelial growth factor (VEGF). Immunohistochemical MaxVision method was adopted to detect the expression level of AEG-1, NF-kB p65 and VEGF in 45 samples of invading cervical cancer and 12 samples of cervicitis from The First Affiliated Hospital of Wenzhou Medical University. Tumor microvascular endothelial marker CD34 combined with Weidner was used to determine the MVD in cervical cancer tissue. The positive expression and staining conditions of AEG-1, NF-kB p65 and VEGF in cervical cancer tissues were observed under a light microscope. Correlations between expression of AEG-1 protein and those of NF-Kb p65 and VEGF, as well as MVD, were analyzed using Pearson correlation. The expression levels of AEG-1 were 0.186±0.043 in cervical cancer and 0.051±0.002 in chronic cervicitis (p<0.01). Moreover, expression of AEG-1 was related to vascular invasion and lymphatic metastasis of cervical cancer (p<0.01), but not with age of the patients, differentiation degree, tumour size, pathological type and parametrial infiltration (p>0.05). Pearson correlation analysis showed that the expression of AEG-1 was linked with NF-kB p65 (r=0.501, p=0.000), VEGF (r=0.718, p=0.000) as well as MVD in cervical cancer tissue (r=0.815, p=0.000). AEG-1 is highly expressed in cervical cancer and promotes angiogenesis, which might be related to the fact that AEG-1 activating the signal pathway of NF-kB could up-regulate the level of VEGF expression.
Highlights
Cervical cancer is one of the most common malignant tumors in female and has a tendency to young people in recent years, many of which have bad biological behaviors at an early stage such as early invasion and metastasis, causing unfavorable prognosis (Ferlay et al, 2008; Miglierini et al, 2014)
Pearson correlation analysis showed that the expression of astrocyte elevated gene-1 (AEG-1) was linked with NF-kB p65 (r=0.501, p=0.000), vascular endothelial growth factor (VEGF) (r=0.718, p=0.000) as well as microvascular density (MVD) in cervical cancer tissue (r=0.815, p=0.000)
AEG-1 is highly expressed in cervical cancer and promotes angiogenesis, which might be related to the fact that AEG-1 activating the signal pathway of NF-kB could up-regulate the level of VEGF expression
Summary
Cervical cancer is one of the most common malignant tumors in female and has a tendency to young people in recent years, many of which have bad biological behaviors at an early stage such as early invasion and metastasis, causing unfavorable prognosis (Ferlay et al, 2008; Miglierini et al, 2014). The activated NF-kB, as regulatory factor in the upstream, can un-regulate the expression of the multiple targeted genes in the downstream, producing a variety of functional protein of regulating biological functions of tumor cells such as cell metabolism, survival, apoptosis and angiogenesis (Endad et al, 2009). This study was intended to use tumor microvascular endothelial marker CD34 and immunohistochemical MaxVision method to detect the expressions of AEG-1, NF-kB p65 and VEGF in cervical cancer tissues and analyze the correlation between AEG-1 and angiogenesis of cervical cancer
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
