Apaf-1和AEG-1在结肠癌中的表达及意义

Abstract

Objective To study the expressions of apoptotic protease activating factor-1(Apaf-1) and astrocyte elevated gene-1(AEG-1) in colonic carcinoma, and to explore their correlations with the clinical pathological features. Methods The expressions of Apaf-1 and AEG-1 were detected in 63 colonic carcinoma samples and 30 normal colonic mucosa adjacent to tumor nest by immunohistochemical method, and their correlations with clinical features of colonic carcinoma were analyzed. Results The positive expressions of Apaf-1 and AEG-1 in colonic carcinoma were 23.81% (15/63) and 68.25% (43/63), respectively. The positive expre-ssions of Apaf-1 and AEG-1 in normal colonic mucosa were 76.67% (23/30) and 26.67% (8/30), respectively. The positive expression rate of AEG-1 was significantly higher in colonic carcinoma than that in normal tissue (χ2=14.192, P=0.000). However, the expression of Apaf-1 was signi-ficantly lower in colonic carcinoma than that in normal tissue (χ2=23.497, P=0.000). The expression of Apaf-1 was negatively correlated to the expression of AEG-1 (r=-0.339, P=0.007). The expressions of AEG-1 and Apaf-1 were associated with differentiation degree (χ2=4.643, P=0.031; χ2=12.034, P=0.001) and clinical stage (χ2=6.628, P=0.010; χ2=8.246, P=0.004), but they were not correlated with age (χ2=1.462, P=0.227; χ2=2.401, P=0.121) and tumor size (χ2=0.333, P=0.564; χ2=0.590, P=0.442). Conclusion The expression of AEG-1 is up-regulated in colonic carcinoma, but the expression of Apaf-1 is down-regulated, with a significant negative correlation. Apaf-1 and AEG-1 may be closely related to the occurrence and development of colon carcinoma. Therefore, combination detection of Apaf-1 and AEG-1 may be more valuable for the prognosis evaluation of colonic carcinoma. Key words: Colonic neoplasms; Astrocyte elevated gene-1; Apoptotic protease activating factor-1