Abstract
Objective To investigate the treatment outcome of modified intracytoplasmic sperm injection(ICSI) using zona pellucida(ZP)-bound sperm. Methods 82 patients with less, weak, abnormal sperm disease who were conformed to ICSI, were divided into traditional ICSI group and the group of modified ICSI using ZP-bound sperm according to ICSI case number.The results of normal fertilization rate, cleavage rate, high-quality embryo rate, planting rate, clinical pregnancy rate and early abortion rate were compared. Results The women's age, the sterility year, mature egg rate, normal fertilization rate, cleavage rate in the two groups had no statistically significant differences (all P>0.05). The planting rate and clinical pregnancy rate of the observation group (46.6%, 63.3%) were higher than those of the control group(38.5%, 53.6%), but there were no statistically significant differences(all P>0.05). The using embryo rate and high-quality embryo rate of the observation group (73.9%, 51.0%) were significantly higher than those of control group(65.8%, 38.6%), the differences were statistically significant(χ2=5.84, χ2=11.6, all P<0.05). Conclusion Modified ICSI using ZP-bound sperm can effectively improve the embryos quality in ICSI. Key words: Spermatozoa; Zona pellucida; Intracytoplasmic sperm injection; Embryo culture techniques
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