Abstract
Friend and Murray found that long osmification of mouse epididymis revealed localized osmium reduction in epithelial cells; in this instance, in only the outer cisternae and vesicles of the Golgi apparatus. Although many substances reduce osmium, aldehydes and compounds with multiple double bonds are particularly active. Knowing that retinaldehyde (vitamin A aldehyde) is a highly unsaturated aldehyde we applied their technique to eyes of tadpoles of two amphibians (Xenopus laevis and Hyla regilla) and to adult eyes of one pulmonate snail (Helix aspersa). Eyes were extirpated, immersed in unbuffered 2% aqueous osmium tetroxide for two or three days at 40°C, and prepared for electron microscopy by rapid dehydration with ethanol, embedment in Epon, and examination of ultrathin sections without further staining.
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