Abstract
In the present work, the two main factors affecting enzymatic stability, i.e. pH and temperature, were analysed in order to determine the optimum ones to maintain ligninase (LiP) and manganese-dependent peroxidase (MnP) activities for prolonged periods of time. The optimum pH and temperature range obtained was around 4.2 and 34 °C for the former and 4.5 and 32 °C for the latter. Under these conditions LiP and MnP showed a half-life time of about 100 and 500 h, respectively. In addition, extracellular liquid containing mainly MnP (200 U/l) was able to decolorize about 20% of the polymeric dye Poly R-478 in 15 min. The decolorization was carried out at a pH of 4.5 (6 mM sodium malonate) and a temperature of 30 °C.
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