Extracellular ligninolytic enzyme production by Phanerochaete chrysosporium in a new solid-state bioreactor

Abstract

The production of manganese-dependent peroxidase (MnP) and lignin peroxidase (LiP) by the fungus Phanerochaete chrysosporium (ATCC 24725) in a new bioreactor, the Immersion Bioreactor, which grows cells under solid-state conditions, was studied. Maximum MnP and LiP activities were 987 U l−1 and 356 U l−1, respectively. The polymeric dye, Poly R-478, was degraded at 2.4 mg l−1 min−1 using the extracellular culture filtrate.