Abstract
Centella asiatica, a medicinal herb consumed as a food supplement and used in traditional and folklore medicine is being overexploited for its therapeutic properties. Lack of adequate cultivation and replacement practices could engender the depletion of the wild variety. Application of plant tissue culture has helped in the regeneration and preservation of several plants in-vitro. The present study reports media standardization for micropropagation and callus induction for the conservation of Centella asiatica using field-grown plants. In media standardization for callus induction, appreciable results were obtained where full strength MS (Murashige and Skoog) media was supplemented with 2 mg.L-1 IAA (Indole acetic acid) and a combination of 0.5 mg.L-1 Kn (Kinetin) with 1 mg.L-1 IAA while MS media supplemented with a combination of 0.5 mg.L-1 Kn and 1.5 mg.L-1 IAA was used for callus proliferation. Combination of BAP (6- Benzyl amino purine) and IAA from preliminary experimentation on micropropagation showed high shooting frequency and were further optimized with parameters such as sucrose and incubation period using Response surface methodology (RSM) to study their individual and interactive effects. A maximum shoot height of 3.8 cm was obtained from the optimized values after 13 days with both BAP and IAA at 0.5 mg.L-1 and sucrose at 6% with the R2 value at 0.73.
Highlights
Centella asiatica is a small herbaceous annual plant of the family Apiaceae and has been in use as a medicinal herb for thousands of years in folklore medicine and Ayurveda, the Indian system of medicine
The present study reports media standardization for micropropagation and callus induction for the conservation of Centella asiatica using field-grown plants
Response surface methodology (RSM) is a collection of mathematical and statistical techniques useful for the modelling and analysis of a process, in which a desired response is affected by several independent factors and the main objective is to optimize this response by optimizing the factors
Summary
Centella asiatica is a small herbaceous annual plant of the family Apiaceae and has been in use as a medicinal herb for thousands of years in folklore medicine and Ayurveda, the Indian system of medicine. A standard RSM design called central composite design (CCD) is the most popular technique utilized to design the experiments These optimization techniques are usually employed once the important factors and their concentration ranges have been identified from preliminary analysis and further optimization is required within the defined factors and concentration range for the desired response (Sagwan et al, 2011). In this present study, growth parameters and regulators were selected after preliminary studies and optimized for shoot initiation of C.asiatica using Central composite design. Media standardization was performed for callus induction, maintenance and proliferation of C.asiatica which could be used for plant regeneration
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
