Abstract
Objective To optimize extraction technology of Fufang-Fuqing-Xiji. Methods Orthogonal test combined with Plackett-Burman experimental design was adopted to study the influence factors of water extraction in Fufang-Fuqing-Xiji. The contents of chlorogenic acid, paeoniflorin and caffeic acid in Fufang-Fuqing-Xiji were determined by HPLC gradient elution method at the same time. A multi-index comprehensive score method was applied to analyze the data and to screen the optimum extraction technology. Results The best extraction conditions of Fufang-Fuqing-Xiji were as following: threaded 80 mesh sieve, soaked 2 h, decocted 3 times with 12 times the amount of water for 1h each time. Conclusions This optimized extraction technology was rational and stable, which could be used for the extraction of Fufang-Fuqing-Xiji. Key words: Extraction; Orthogonal test; Plackett-Burman experimental design; Multi-index comprehensive score
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