Research on the extracting process of alkannin from radix arnebiae seu lithospermi

Abstract

Objective Optimizing the preparation technology of Red buttock ointment,by exploring the best extraction conditions of the alkannin, radix arnebiae seu lithospermi polysaccharides and so on. Methods Selecting radix amebiac seu lithospermi 1kg and using orthogonal experiment to explore the best extraction conditions. Naphtho- quinone pigment chemicals were extracted by reflux extraction method;ethanol and paraffin wax were chosen as the composite solvent. Radix arnebiae seu lithospermi polysaecharide was extracted by water decocting, which to observe the rationality of process. Results The best extraction condition is A3B2CIDl , including section A： ethanol was 20 times the quality of medicinal materials （select radix arnebiae seu lithospermi lkg and ethanol 20kg）, section B： the amount of paraffin was 5% of ethanol（select radix arnebiae seu lithnspermi lkg and paraffin 1 kg） ,section C： Boiling water was 20 times the quality of medicinal materials, section D： the boiling time was 2h. Conclusion The more practical production process conditions：Ethanol was 10 times the quality of medicinal materials, and the amount of paraffin was 5% of ethanol. Boiling water was 10 times the quality of medicinal materials, and the boiling time was 1 h. This method is economic, simple, reasonable, stable, reproducible and easy to operation. Red buttock ointment obtained by this method possesses of excellent quality, good performance and superior effect. Key words: Alkannin; Orthogonat Design