Extraction technology optimization of total flavonoids in Wudang pine needle tea by orthogonal test

Abstract

Objective To optimize the best extraction technology of total flavonoids in Wudang pine needle tea. Methods The contents of total flavonoids were taken as index, and orthogonal design of L9(34) was applied to process ultrasonic extraction. Results The order of factors of affecting extraction technology was solid to liquid ratio > ultrasonic power > ultrasonic time. The optimized extraction technology was as follows: adding 500 times volume of purified water, ultrasonic extracting for 15 minutes with ultrasound power 500 W at 40 ℃. The average content of total flavonoids was 39.701 mg/g. Conclusions The optimal extraction technology is simple, efficient and feasible, and can be used for extracting total flavonoids from Wudang pine needle tea, providing basis for the formulation of quality standards. Key words: Orthogonal test; Flavones; Extraction technology; Wudang pine needle tea