Optimization and Production of Industrial Important Cellulase Enzyme from Penicillium citrinum in Western Ghats of Sathuragiri Hills Soil Sample Isolate

Abstract

In optimization of cellulase production by different pH, substrates, nitrogen, carbon sources were detected from penicillium citrinum micro-fungal species. The maximum cellulase activity was recorded in the range of pH- 5 to 6.5 and wheat bran (91 U/ml) and rice bran (81 U/ml) is the best raw substrate for the production of cellulase. Estimation of extracellular protein was recorded in different production medium of Penicillium citrinum (1.63 mg/ml) for maltose followed by sucrose (1.46 mg/ml), fructose (1.13 mg/ml), wheat bran (1.12 mg/ml), peptone (0.88 mg/ml) and yeast extract (0.82 mg/ml). The highest extracellular protein was recorded in pH 5.5 to 6.5 followed by substrate for wheat bran (1.12 mg/ml), rice bran (1.04 mg/ml), paddy straw (1.03 mg/ml). In nitrogen sources, ammonium sulphate (141 U/ml) and ammonium nitrate (121 U/ml) is the best nitrogen source for the production of cellulase enzyme. The maximum cellulase activity was recorded in glucose (134 U/ml) and maltose (159 U/ml) was best carbon source for the production of cellulase.