Optical characterization of polymeric nanocapsules interaction with the cell membrane and in vivo analysis.

Abstract

Polymeric nanocapsules was studied with respect to intracellular drug delivery applications.. Polyelectrolytenanocapsules, fabricated by layer-by-layer (LbL) coating of a sacrificial template followed by the decomposition of this template, have gathered increased interest as novel entities for drug delivery and diagnostic purposes. This permit control of the pharmacokinetics allowing for applications like preventive drug administration or scheduled application of pharmaceuticals during long-term therapy. The planned experiments with various types of payloads providing different molecular weights and zetapotentials, demonstrated a flexible way of tailoring the loading delivery time via the molecular properties of the cargo. But we lost information about the structure of cells changes after interaction with capsules. By diffraction phase microscopy it is possible to assess the morphology of cells in real time. The main obstacle in retrieving quantitative phase with high sensitivity is posed by the phase noise due to mechanical vibrations and air fluctuations that typically affect any interferometric system. In this paper, we review diffraction phase microscopy (DPM), which is a common-path quantitative phase imaging (QPI) method that significantly alleviates the noise problem. Diffraction phase microscopy (DPM), an effective quantitative phase microscopy tool was used for determination cells structure after interaction with the fabricated capsules