A spatial carrier dynamic quantitative differential phase imaging method

Abstract

Differential interference contrast (DIC) microscopy is an important method for quantitative phase imaging (QPI) due to its high compatibility regarding to partial coherent light illumination and excellent optical sectioning ability. However, limited by the on-axis interferometric configuration of Nomarski DIC microscopic imaging system, it is difficult to achieve single-shot phase retrieval. Here, we propose a simple and efficient method for dynamic quantitative differential phase imaging (QDPI) by introducing a spatial carrier device into DIC microscope. A Wollaston prism (WP) is placed on the imaging plane of DIC microscope and then projected by a 4f imaging system, so two orthogonally polarized imaging beams with a certain shearing amount will interfere on the conjugate plane of the 4f imaging system and form a spatial carrier DIC image, thus the QDPI can be conveniently realized. Importantly, the system not only possesses high temporal phase stability provided by the common-path geometries and low spatial phase noise due to partially coherent light illumination, but also the advantages of simple optical implementation, no additional phase distortion, high light energy utilization efficiency, and high signal-to-noise ratio. The experimental result demonstrates that using the proposed method, the phase measurement sensitivity reaches 0.003 rad, the corresponding applicability is verified by a polystyrene microsphere crown and biological cells.