Abstract
Lipases with unique characteristics are of value in industrial applications, especially those targeting cost-effectiveness and less downstream processes. The aims of this research were to: (i) optimize the fermentation parameters via solid state fermentation (SSF); and (ii) study the performance in hydrolysis and esterification processes of the one-step partially purified Schizophyllum commune UTARA1 lipases. Lipase was produced by cultivating S. commune UTARA1 on sugarcane bagasse (SB) with used cooking oil (UCO) via SSF and its production was optimized using Design-Expert® 7.0.0. Fractions 30% (ScLipA) and 70% (ScLipB) which contained high lipase activity were obtained by stepwise (NH4)2SO4 precipitation. Crude fish oil, coconut oil and butter were used to investigate the lipase hydrolysis capabilities by a free glycerol assay. Results showed that ScLipA has affinities for long, medium and short chain triglycerides, as all the oils investigated were degraded, whereas ScLipB has affinities for long chain triglycerides as it only degrades crude fish oil. During esterification, ScLipA was able to synthesize trilaurin and triacetin. Conversely, ScLipB was specific towards the formation of 2-mono-olein and triacetin. From the results obtained, it was determined that ScLipA and ScLipB are sn-2 regioselective lipases. Hence, the one-step partial purification strategy proved to be feasible for partial purification of S. commune UTARA1 lipases that has potential use in industrial applications.
Highlights
Filamentous fungi are suitable for solid state fermentation (SSF) due to their substrate colonizing mycelium, limited water tolerance and extracellular enzyme production
Singh et al [3] has purified the lipase produced by S. commune which was cultivated on Leucaena leucocephala seeds under solid state fermentation conditions
The potential applications of ScLipA and ScLipB in their partial purified forms were presented in this study
Summary
Filamentous fungi are suitable for solid state fermentation (SSF) due to their substrate colonizing mycelium, limited water tolerance and extracellular enzyme production. Schizophyllum commune is a commonly distributed split gill white-rot mushroom [1,2] found worldwide. It is cultivated in Malaysia, as it is popularly eaten by the Malay community [1]. Singh et al [3] has purified the lipase produced by S. commune which was cultivated on Leucaena leucocephala seeds under solid state fermentation conditions. Due to these findings, S. commune was selected for this study
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