Abstract
The standard preparative procedure for soluble nucleohistone 1 was carefully examined. A critical stirring speed was identified at which nucleohistone is invariably completely soluble. The time of the preparation was reduced by replacing the dialysis step by the addition of salt. It was demonstrated that the procedure could be adapted to preparing soluble nucleohistone above its insolubility salt range, in different stages of histone dissociation by salt. A sedimentation investigation of soluble nucleohistone, at these different stages of histone dissociation, provided evidence for a substantial conformational change in the nucleohistone compatible with the loss of supercoiled structure.
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