Abstract
The biosynthesis of 26-S and 17-S ribosomal RNA (rRNA) in the yeast Saccharomyces carlsbergensis was investigated. Four high-mol. wt. ribosomal RNA precursors with a size corresponding to sedimentation values of 42 S, 37 S, 32 S and 29 S were found to be present in this organism. These ribosomal precursor RNA's were purified and isolated by means of polyacrylamide gel electrophoresis of the nuclear RNA fraction. Structural comparison between the four purified ribosomal RNA precursors, pulse-labeled in vivo with various radioactive markers and the two rRNA components was made by competitive hybridization with homologous DNA. These studies showed that the ribosomal RNA precursors contain, besides sequences of mature rRNA, also nonribosomal stretches which are not conserved during the processing of 42-S RNA into rRNA but are removed in a number of steps. On the basis of these findings and the determined molecular weights of the four ribosomal RNA precursors and the two rRNA's, a scheme for the biosynthesis of rRNA in yeast is proposed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
