Oligomeric Proanthocyanidins Alleviate the Detrimental Effects of Dietary Histamine on Intestinal Health of Juvenile American Eels (Anguilla rostrata)

Abstract

This study evaluated the oligomeric proanthocyanidins (OPC) on alleviating the detrimental effects in the intestine caused by dietary histamine in juvenile American eels (Anguilla rostrata). A total of 480 fish with a similar body weight of 10.84 ± 0.16 g were randomly divided into four groups, including the control group fed a basal diet, the HIS group fed a diet with a high level of histamine (534 mg/kg), the H + OPC I group fed the high histamine diet with 300 mg/kg OPC, and the H + OPC II group fed the high histamine diet with 600 mg/kg OPC. After the fish were fed the trial diets for 77 days, the intestinal samples were taken, and the related parameters were analyzed. Dietary 300 mg/kg or 600 mg/kg OPC could reverse the decreased activities of lipase, protease, and glutathione peroxidase and the level of total antioxidant capacity; the increased intestinal malondialdehyde and D-lactate acid levels and the activity of diamine oxidase in serum; and the decreased villus height caused by a high level of dietary histamine. There were no significant differences above all the indices between the H + OPC I group and the H + OPC II group. The higher relative abundances of potentially pathogenic bacteria were induced by the high level of dietary histamine. Dietary 300 mg/kg OPC might increase the relative abundance of the potential probiotics and inhibit the colonization of intestinal pathogenic bacteria. The intestinal health status of the H + OPC groups was similar to that of the control group. These results suggested that dietary 300 mg/kg OPC might alleviate the detrimental effects of dietary 534 mg/kg histamine on the intestine health of juvenile American eels by increasing the activity of digestive enzymes, improving the antioxidative potential and barrier function, and beneficially modulating the intestinal microbiota. Dietary 600 mg/kg OPC could not exert further improvement in growth performance and the intestinal health of juvenile American eels.