Effect of dietary histamine supplementation on growth, digestive enzyme activities and morphology of intestine and hepatopancreas in the Chinese mitten crab Eriocheir sinensis.

Liulan Zhao,Song Yang,Xiaozhen Yang,Yongxu Cheng

doi:10.1186/s40064-016-2105-9

Liulan Zhao, Song Yang + Show 2 more

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https://doi.org/10.1186/s40064-016-2105-9

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Abstract

A 28-days feeding experiment was conducted to investigate the effect of histamine on digestive physiology of the Chinese mitten crab, Eriocheir sinensis. Four experimental diets were supplemented with histamine at 0, 1, 2, 4 g/kg. Histamine supplementation had no effect on growth. The activities of digestive enzyme decreased significantly at first (days 7 and 14) (p < 0.05) and then increased or finally slightly recovered in the hepatopancreas and intestinal tract on the 28th day. Tryptase and amylase activities were inhibited significantly in each histamine-treated group on day 7 as compared to the control (p < 0.05). On day 7, 14 and 28, tryptase mRNA relative expression in the histamine treatments correlated positively with the histamine concentration (p < 0.05). Histopathologic analyses showed serious alterations in hepatopancreas, moderate alterations in the hindgut and intestinal bulb, and no alterations in the midgut. In hepatopancreas, low levels (1 g/kg) of histamine caused an increase in the number of B-cells. High levels (4 g/kg) of histamine increased the number of R-cells, which were also highly vacuolized. In extreme cases, the basal lamina was detached from the tubule. In the intestinal bulb and hindgut, high levels of histamine (4 g/kg) decreased the density of reserve inclusion cells. Thus, this indicated that histamine had dose-dependent effect on the activity of digestive enzymes and the morphology of the intestine and hepatopancreas.

Highlights

The Chinese mitten crab (Eriocheir sinensis) is a native freshwater crab throughout the eastern region of China (Sui et al 2009)
Changes of digestive enzyme activities in the hepatopancreas and intestinal tract tissue during the period of experiment In the hepatopancreas The results indicated that dietary histamine inhibited tryptase activity in the hepatopancreas during the Forward primer Reverse primer β-Actin-F β-Actin-R
We have demonstrated that the activities of digestive enzymes decreased significantly at first (p < 0.05) and increased or slightly recovered in the hepatopancreas and intestinal tract after feeding dietary histamine for 28 days

Summary

Introduction

The Chinese mitten crab (Eriocheir sinensis) is a native freshwater crab throughout the eastern region of China (Sui et al 2009). The effect of dietary histamine on digestive physiology of aquatic animals is still unclear. When fed diets supplemented with histamine at 2 g/ kg, a decline in feed consumption in the rainbow trout (Oncorhynchus mykiss) occurred (Fairgrieve et al 1998). The reports are limited on the histopathology and enzymology of the digestive system after feeding dietary histamine in aquatic animals. Fairgrieve et al (1994) reported that histamine supplementation of 2 g/kg caused intestinal damage in rainbow trout, including distended stomachs and stomach erosion. Watanabe et al (1987) documented the effect of histamine on the formation of gastric lesions in rainbow trout (Watanabe et al 1987; Fairgrieve et al 1994). No further information about the effect of histamine on digestive physiology can be found in any other aquatic animals

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