Abstract
A TLC procedure was developed for the separation of phenoxazinone-type pigments. By employing silica gel G as the adsorbent and a solvent system of benzene-ethyl acetate-glacial acetic acid-formic acid (12:6:1:1), the separation of the following reference compounds was accomplished: cinnabarinic acid (Rf 0.35), cinnabarin (0.47), 2-amino-3-oxo-3H-phenoxazine (0.57), tramesanguin (0.67), and phenoxazine (0.99). Examination of the individual acetone extracts of Pycnoporus cinnabarinus (Jacq. ex Fr.) Karst., P. coccineus (Fr.) Bond. and Sing., and P. sanguineus (L. ex Fr.) Murr. by this technique revealed that all three species contained the same pigments. In addition to cinnabarinic acid, cinnabarin, and tramesanguin, two additional phenoxazinone-type pigments (Rf 0.53 and 0.90) were found in the respective carpophores.
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