Abstract
AbstractImazalil, incorporated in a solid nutrient medium, had no effect on the sporulation of Penicillium italicum at concentrations that did not strongly inhibit mycelial growth. However, when the fungus was grown submerged in liquid culture, sporulation of P. italicum appeared to be more affected than vegetative growth; at 33 nM, imazalil completely prevented the formation of penicilli without adverse effects on growth. Imazalil was able to prevent sporulation in the vapour phase as shown by scanning electron microscopy on P. italicum and Aspergillus niger; the vapours of the compound were found to affect different stages of the sporulation process, depending on the timing of the experiment. Thus, in order to show antisporulant activity, imazalil had to reach the target site by means other than migration within the fungus.
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