Abstract
In recent years, marked increase in the number of drug resistance (DR)of M. tuberculosis (MBT) to rifampicin (Rif), which is a marker of MDR. Aim: to study efficacy of definition of susceptibility MBT to Rif accelerated methods: Xpert® MTB/Rif (Meth. 1), BACTEC MGIT 960 (Meth. 2) compared with traditional method of seeding on solid nutrient media . Materials . Study 83 patients with newly diagnosed pulmonary tuberculosis (TB) with high risk DR. Investigated parallel sputum, Meth. 1, Meth. 2 and by seeding on solid nutrient media. Results: DR MBT to Rif discovered by Meth. 1 in 30 (36.2%), by Meth. 2 in 29 (34.9%), of these MDR in 28 (33.7%) and isolated Rif resistance in 1 (1.2%) patients. DR MBT to Rif discovered by method by seeding on solid nutrient media. - in 28 (33.7%), including MDR-25 (30.1%) and isolated Rif resistance in 3 (3.6%) patients. Coincidence between results of research by Meth. 1 and by seeding on solid medium for strains sensitive to Rif was 100% for resistant to Rif 98,9%. Meth. 1 compared with seeding on solid media received 2 false-positive results. Coincidence of results of research on Meth. 2 and culture on a solid medium that are sensitive to Rif was 100%, resistant strains Rif 94.5%. Meth. 2 obtained 1 false-negative and 2 false-positive results. Conclusions: Meth. 1 and 2 showed a high percentage of matches with method of seeding on solid nutrient media for DR to Rif 98,9% and 94,5%, respectively. Sensitivity of Meth. 1 is 100%, specificity - 96.4%, Meth. 2 sensitivity - 98.6%, specificity - 96.4%.
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