Abstract
Summary Carbon and nitrogen sources and pH range controlling growth of Rhizobium sesbaniae localy isolated from root nodules of S. sesban (L) Merrill were studied in vitro. Some factors affecting extracellular invertase activity of the bacterium were investigated. The isolated strain showed excellent growth on most of carbon sources tested, while it cannot grow on either starch or cellulose. The survey of nitrogen sources showed that uracil and phenyl alanine had a stimulatory effect, while ammonium chloride and adenine inhibited the growth of the bacterium. The minimum, optimum and maximum pH values affecting the growth of the isolate were 2, 8 and 10 respectively. The invertase activity of R. sesbania was optimum after six days of incubation, pH 7 and 15 g/l sucrose in growth culture.
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