Abstract
Fusarium solani NVS671 identified from infected sugarcane stem of Co 671 as a new pathogen was subjected to various cultural conditions to understand its physiological profile. In continuation with our previous work, cultural analysis was carried out under in vitro condition by supplementing various carbon and nitrogen sources in Czapek-Dox agar (CDA). Under different hydrogen ion concentrations, it was found that the growth of Fusarium solaniNVS671 was less at pH 4 and pH 10 and could reach up to 5 cm after 7 days of incubation on Potato Dextrose Agar (PDA). It is observed that the pH around 7 to 8 was optimum for the growth of F. solani. Ten different nitrogenous (N) sources and nine different carbon sources were tested on CDA medium to know their effect on the mycelial growth rate and characteristics of the fungus. Among the N sources evaluated, ammonium chloride (7.96±0.11 cm) was found to be most efficient for mycelial growth promotion followed by ammonium nitrate (7.7±0.1 cm) and ammonium sulphate (7.3±0.1 cm). The most preferred carbon source recorded to promote best radial mycelial growth was starch (7.96±0.05 cm) and sucrose (7.93±0.05 cm). Capabilities of using different carbon and nitrogen sources and ability to grow at different pH levels may allow species to adapt to specific soil conditions.This study is important to understand the physiology and metabolite preference of F. solani.
Highlights
Fusarium solani (Teleomorph: Haematonectria haematococca) is a widely distributed fungal plant pathogen that infects many different genera of plants [1, 2]
In our previous study we have reported that F.solani NVS671 is a new pathogen found to causing stem rot in sugarcane [10]
Effect of pH on mycelial growth was studied by growing F.solani NVS671 on Potato Dextrose Agar (PDA) mixed with an appropriate volume of the buffer to give desired pH in medium
Summary
Fusarium solani (Teleomorph: Haematonectria haematococca) is a widely distributed fungal plant pathogen that infects many different genera of plants [1, 2]. F. solani and R. solani are the most important saprophytic pathogens, which develop in both cultured and non-cultured soils, causing the symptoms of damping off and root rot diseases to wide range of vegetable and crop plants including tomato [5]. We analysed effect of different pH, carbon and nitrogen substrates in PDA and CDA medium on the growth of F.solani NVS671. Effect of pH on mycelial growth was studied by growing F.solani NVS671 on PDA mixed with an appropriate volume of the buffer to give desired pH in medium. To scrutinize the effect of different carbon and inorganic nitrogen sources on growth and cultural characteristics of fungal pathogen Czapek Dox agar (CDA) media were used. In case of pH analysis the significance of differences between the treatments was evaluated by one way analysis of variance (ANOVA) at the significance level of 95 %
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