Novel Proniosomes of Manidipine: Optimization and In-vivo Evaluation

Abstract

The objective of the current study is to produce proniosomes of manidipine using the thin film hydration method for the management of hypertension. Statistical optimization was performed using the Box-Behnken design. The generated formulations were tested for particle size, entrapment effectiveness, and drug release at 12 hours. The improved proniosomes were further assessed for surface morphology, ATR, and differential scanning calorimetry (DSC). The improved proniosomes were also assessed for an in-vitro and in-vivo investigation on dexamethasone-induced hypertension. It was noted that there was no significant interaction with excipients. DSC indicated a considerable shift in the endothermal peak and showed interaction with the study’s excipients. Niosomes and drug crystals were seen to be dispersed in small areas during the scanning electron microscope (SEM) analysis. The study also showed that the optimized proniosomes have asymmetrical surfaces and appearances. The zeta value in the research was -6.7 mV, indicating stability. According to the optimized proniosomes formulation, F14 released 99.97% of the drug at its highest concentration in 12 hours. ANOVA results from an in-vivo investigation confirmed a satisfactory outcome in decreasing raised blood pressure, as shown by the F and p-values.