Novel fabrication and development of multifunctional Zn/Fu@Cs nanofibers material for wound care and operate room infection control

Abstract

Treatment of operate room wounds infections is a clinically more challenging process. Therefore, several techniques and wound care materials have been taken to improve wound healing mechanisms. The present study is mainly focused on zinc oxide and fucoidan-loaded chitosan nanofiber scaffolds fabricated by the electrospinning method. The functional groups, morphology, and hydrothermal stability of fabricated Zn/Fu@Cs were observed and investigated by microscopic and some spectroscopic techniques. The wound healing potential of Zn/Fu@Cs nanofiber has been evaluated by various in vitro biological experiments. In addition, the prepared nanofiber showed suitable bacterial growth inhibition against P. aeruginosa, B. subtilis, S. aureus, and E. coli wound infecting bacteria and in vitro studies confirmed the excellent cell proliferation, and cytocompatibility. In vitro study exhibited significant cell proliferation and viability is observed in Zn/Fu@Cs nanofiber treated L929 cells within 3 days, which is comparable to the control it is higher. In wound scratch assay the wound healing efficiency has been monitored on the human skin fibroblast L929 cell line. The wound scratch experiment results revealed that the Zn/Fu@Cs nanofiber shows quick cell regeneration without bacterial infections. The biodegradation study concluded the biocompatibility of nanofibers under physiological condition. The overall results suggest that the Zn/Fu@Cs nanofiber is a potential material for wound care with enhanced antibacterial property against operate room pathogens.