Abstract
We demonstrate that two-beam interferometry is applicable to phase detection of nonlinear susceptibility of biological samples in the stimulated parametric emission (SPE) microscopy, which is one of the coherent nonlinear optical microscopy techniques. We experimentally observed stained mouse kidney cells and successfully confirmed nonlinear phase shift associated with an electronic resonance at stained nuclei. In addition, phase blurring was found to be almost completely eliminated in a nonlinear differential interferometric contrast image, which can be produced through a simple post-processing procedure.
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