Abstract
Two types of newly developed nonlinear-optical microscopy are presented, namely, stimulated parametric emission (SPE) microscopy and stimulated Raman scattering (SRS) microscopy, together with their biological applications. These techniques allow high-resolution 3D visualization of unstained living cells. SPE microscopy is also applied to time-lapse observation of dividing cells as well as simultaneous observation of fluorescence emitted from labeled organelles and SPE signal.
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