Non-contiguous finished genome sequence and description of Bacillus massiliogorillae sp. nov.

Mamadou Bhoye Keita,Fadi Bittar,Pierre-Edouard Fournier,Didier Raoult,Catherine Robert,Seydina M. Diene

doi:10.4056/sigs.4388124

Mamadou Bhoye Keita, Fadi Bittar + Show 4 more

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https://doi.org/10.4056/sigs.4388124

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Abstract

Strain G2T sp. nov. is the type strain of B. massiliogorillae, a proposed new species within the genus Bacillus. This strain, whose genome is described here, was isolated in France from the fecal sample of a wild western lowland gorilla from Cameroon. B. massiliogorillae is a facultative anaerobic, Gram-variable, rod-shaped bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 5,431,633 bp long genome (1 chromosome but no plasmid) contains 5,179 protein-coding and 98 RNA genes, including 91 tRNA genes.

Highlights

Strain G2T (= CSUR P206 = DSM 26159) is the type strain of B. massiliogorillae sp. nov. This bacterium is a Gram-variable, facultatively anaerobic, indolenegative bacillus having rounded-ends. It was isolated from the stool sample of Gorilla gorilla gorilla as part of a “culturomics” study aiming at cultivating bacterial species within gorilla feces
The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA similarity to other members of the genus Bacillus, and is part of a “culturomics” study of the gorilla flora aiming at isolating all bacterial species within gorilla feces
B. massiliogorillae has a lower G+C content than B. psychrosaccharolyticus (34.95% vs 38.8%) and B. megaterium (34.95% vs 38.1%) but slightly higher than that B. thuringiensis (34.95% vs 34.8%)

Summary

Introduction

The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA similarity to other members of the genus Bacillus, and is part of a “culturomics” study of the gorilla flora aiming at isolating all bacterial species within gorilla feces. It was the 61st genome of a Bacillus species and the first genome of Bacillus massiliogorillae sp. Strain G2T and another 3 Bacillus species (Table 6), we compared genomes pairwise and determined the mean percentage of nucleotide sequence identity among orthologous ORFs using BLASTn. Orthologous genes were detected using the Proteinortho software [34]. 100 1.84 98.63 73.39 75.49 11.78 26.01 a The total is based on either the size of the genome in base pairs or the total number of protein coding genes in the annotated genome

Cell motility

Findings

Conclusion