Non-contiguous finished genome sequence and description of Paenibacillus gorillae sp. nov.

Mamadou Bhoye Keita,Roshan Padhmananabhan,Fadi Bittar,Pierre-Edouard Fournier,Aurélia Caputo,Eric Delaporte,Didier Raoult,Catherine Robert

doi:10.4056/sigs.5189179

Abstract

Strain G1T sp. nov. is the type strain of Paenibacillus gorillae a newly proposed species within the genus Paenibacillus. This strain, whose genome is described here, was isolated in France from the fecal sample of a wild western lowland gorilla from Cameroon. P. gorillae is a facultative anaerobic, Gram-negative, rod-shaped bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 6,257,967 bp long genome (one chromosome but no plasmid) contains 5,856 protein-coding and 62 RNAs genes, including 60 tRNA genes.

Highlights

Strain G1T (= CSUR P205 = DSM 26181) is the type strain of Paenibacillus gorillae sp. nov. This bacterium is a Gram-negative, flagellated, facultative anaerobic, indole-negative bacillus that has rounded-ends. It was isolated from the stool sample of Gorilla gorilla subsp. gorilla as part of a culturomics study aiming at cultivating bacterial species found within gorilla feces
By applying large-scale culture conditions, culturomics has previously facilitated the isolation of many new bacterial species from human stool samples [1,2,3]
Genome project history The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA similarity to other members of the genus Paenibacillus, and is part of a “culturomics” study of the gorilla flora which aims to isolate all bacterial species within gorilla feces

Summary

Introduction

Strain G1T (Table 1) was isolated in January 2012 by cultivation on Columbia agar with sheep blood 5% (BioMerieux, France) This strain exhibited a 98.28% 16S rRNA nucleotide sequence similarity with Paenibacillus xinjiangensis, the phylogenetically closest validly published Paenibacillus species (Figure 1). Genome project history The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA similarity to other members of the genus Paenibacillus, and is part of a “culturomics” study of the gorilla flora which aims to isolate all bacterial species within gorilla feces. Genome sequencing and assembly A shotgun and a 3 kb paired end library were pyrosequenced on the 454_Roche_Titanium. This project was loaded on a 1/4 region for each application on PTP Picotiterplates. AThe total is based on either the size of the genome in base pairs or the total number of protein coding genes in the annotated genome

5.79 Function unknown

Findings

Conclusion