Abstract
Strain G4(T) was isolated from the stool sample of a wild gorilla (Gorilla gorilla gorilla) from Cameroon. It is a facultative anaerobic, Gram-negative, rod-shaped bacterium. This strain exhibits a 16S rRNA nucleotide sequence similarity of 97.48% with Paenibacillus typhae, the phylogenetically closest species with standing nomenclature. Moreover, the strain G4(T) presents some phenotypic differences when compared to other Paenibacillus species and shows a low MALDI-TOF Mass Spectrometry score that does not allow any identification. Thus, it is likely that this strain represents a new species. Here, we describe the characteristics of this organism, complete genome sequence, and annotation. The 6,933,847 bp size genome (1 chromosome but no plasmid) contains 5972 protein-coding genes and 54 RNAs genes, including 44 tRNA genes. In addition, digital DNA-DNA hybridization values for the genome of the strain G4(T) against the closest Paenibacillus genomes range between 19.7 and 22.1, once again confirming its new status as a new species. On the basis of these polyphasic data, consisting of phenotypic and genomic analyses, we propose the creation of Paenibacillus camerounensis sp. nov. that contains the strain G4(T).
Highlights
Strain G1T (= CSUR P205 = DSM 26181) is the type strain of Paenibacillus gorillae sp. nov
Genome project history The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA similarity to other members of the genus Paenibacillus, and is part of a “culturomics” study of the gorilla flora which aims to isolate all bacterial species within gorilla feces
P. gorillae has a lower G+C content than those of “G. massiliense” and P. elgii (48.8% vs 50.39% and 52.6% respectively) but higher than those of P. alvei and B. brevis (48.8% vs 45.9% and 47.3% respectively) and slightly higher than P. massiliensis (48.8% vs 48.5%)
Summary
Strain G1T (Table 1) was isolated in January 2012 by cultivation on Columbia agar with sheep blood 5% (BioMerieux, France) This strain exhibited a 98.28% 16S rRNA nucleotide sequence similarity with Paenibacillus xinjiangensis, the phylogenetically closest validly published Paenibacillus species (Figure 1). Genome project history The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA similarity to other members of the genus Paenibacillus, and is part of a “culturomics” study of the gorilla flora which aims to isolate all bacterial species within gorilla feces. Genome sequencing and assembly A shotgun and a 3 kb paired end library were pyrosequenced on the 454_Roche_Titanium. This project was loaded on a 1/4 region for each application on PTP Picotiterplates. AThe total is based on either the size of the genome in base pairs or the total number of protein coding genes in the annotated genome
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