Abstract
Multiple sclerosis (MS) is a neurodegenerative disease with various factors affecting its etiology. Overproduction of nitric oxide and subsequent lesions of biopolymers are some of the possible causes of the disease. This study aimed to measure the most relevant nitrosative and oxidative stress biomarkers and the level of modified DNA bases in patients with MS. Each parameter was assayed in 25 patients with MS and 25 healthy controls. This study involved detecting blood plasma and serum nitric oxide metabolites by chemiluminescence detector Sievers NOA-280i, malondialdehyde (MDA) measurements with thiobarbituric acid reactive substance (TBARS) assay, detection of oxidized purines and pyrimidines with the enzyme-modified comet assay. Statistical analysis of the results was performed by one-way analysis of variance (ANOVA) and unpaired t test for the comparison of less than three data sets. DNA single-strand breaks, levels of modified purines and pyrimidines, as well as nitrite and nitrate levels in plasma and serum samples, were significantly higher in patients with MS than in healthy controls. On the contrary, MDA levels appeared to be lower in patients with MS.
Highlights
Multiple sclerosis is a neurodegenerative disease with chronic and inflammatory characteristics
The present study aimed to evaluate the oxidative stress damage in peripheral blood mononuclear cells, measurements of NO metabolite, and lipid peroxidation products in human plasma and serum
The study group consisted of 25 unrelated patients with Multiple sclerosis (MS) randomly chosen among the MS patients attending the Latvian Maritime Medicine Center and the control group of 25 healthy subjects: 4 males and 21 females of 20 to 43 years
Summary
Multiple sclerosis is a neurodegenerative disease with chronic and inflammatory characteristics. MS targets axons in the CNS, causing their demyelination, disrupting signaling pathways (Stadelmann et al, 2011; Trapp and Nave, 2008). The exact cause of the demyelination is still unknown. This process is supposed to develop as the sequence of various factors such as environmental, genetic, metabolic, viral, or oxidative stress-induced. Together or separately, these factors result in an autoimmune disorder with the consequent immune attack on the CNS (Fiorini et al, 2013; Gonsette, 2008; Miller et al, 2012)
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