Đánh giá kết quả nhuộm hóa mô miễn dịch thủ công so với nhuộm tự động thụ thể estrogen trong ung thư vú

Abstract

Objectives: Determine the rate and extent of ER expression in BC by manual IHC staining method compared with automatic machine staining; Comment on some factors affecting the quality of manual IHC staining with ER compared to automatic machine staining. Subjects: 33 cases of BC and results of IHC test with ER+ marker at K1 hospital in 2022. Research method: Experimental research. Results: With IHC staining by automatic machine, the positive rate is 100%, of which positive 2(+) accounts for the highest rate with 51.5%, then positive 3(+) and positive 1(+) is 39.4 and 9.1%, respectively. With manual IHC staining, the positive rate is 97%, of which positive 2(+) also accounts for the highest rate with 54.5%, then positive 3(+), positive 1(+) and negative (0) was 36.4, 6.1 and 3%, respectively. The expression level of ER in manual IHC staining is weaker than in automatic machine staining. The degree of compatibility between the two methods is quite correlated (Cramer's V = 0.967 with p < 0.001). Conclusion: IHC staining with ER by automatic machine has a higher positive rate, stronger expression intensity, and a higher percentage of good specimens than in manual IHC staining. Keywords: Breast cancer; Estrogen receptor; Automated Immunohistochemistry; Manual Immunohistochemistry; Endocrine therapy.