Abstract
Medicinal mushroom, Cordyceps militaris, an entomopathogenic fungus with medicinal and commercial values, is over-exploited, leading to scarcity in nature. The cultivation procedure of C. militaris on host under in vitro condition has been carried out successfully. The results showed that SDAY2 media was suitable for fast growing mycelium after 5 days of culture and large fruiting body size, very fine mycelium, cotton and reptile surface. Liquid medium CT1 showed that optimal fungal spore density after 11 days of culture, by injecting 2 mL of fungal liquid into the top of the pupal stage of Brihaspa astrostigmella, Rhynchophorus ferrugineus and Bombyx mori were highly effective, mycelium develops quickly and forms fruiting bodies, infection rate of C. militaris and Bombyx mori productivity the highest (> 95%; length 7.05 cm, diameter 2.31 mm and weight 0.32 g/fruit), cordycepin and adenosine content in the fruiting body dried to 5.25 mg/g and 0.71 mg/g, 6.1 mg/g and 0.52 mg/g, 5.34 mg/g and 0.58 mg/g respectively for Brihaspa astrostigmella, Rhynchophorus ferrugineus and Bombyx mori. These techniques can be applied to produce C. militaris mushroom on hosts to meet the market demand for current Cordyceps products.
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