Abstract
Selenium is an essential micronutrient for human, animal, and microorganism health. The biocompatibility, bioavailability, and low toxicity of selenium nanoparticles (SeNPs) have recently sparked the interest of scholars. Electrochemical plasma is a novel electrical discharge in a liquid environment, such as an aqueous solution or an organic compound. In this study, electrochemical plasma was utilized to synthesize SeNPs with a size range of 60 to 80 nm in water ethanol mixtures. UVVisible Spectrophotometry (UV-Vis), X-Ray Diffraction (XRD), Raman spectroscopy, Scanning Electron Microscope (SEM), Energy dispersive spectroscopy and mapping (EDX), and X-ray photoelectron spectroscopy (XPS) were used to characterize the synthesized SeNPs. The obtained SeNPs showed good antimicrobial properties when testing with bacteria Staphylococcus aureus, Escherichia coli. The SeNPs showed effective bioactivity with inhibition zones of 18, and 25 mm against E. coli, and S. aureus, respectively, at a SeNPs dose of 150 µL (concentration of 300 ppm)
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