Abstract
An amperometric biosensor for the detection of organophosphorus and carbamate type pesticides was developed. Acetylcholinesterase enzyme (electric eel) was immobilized in a thin-layer enzyme cell, and acetylthiocholine chloride (AcTCh) was used as substrate. Amperometric detection with glassy carbon electrode was performed in a stopped-flow FIA system. The inhibition effect of dichlorvos (DDVP), carbofuran, methomyl, and pirimicarb were examined: the linear measuring ranges were 0.001–0.1 μmol l−1, 0.001–0.1 μmol l−1, 0.2-1 μmol l−1, and 0.1–10 μmol l−1, respectively. Soil extract and apple juice were measured with spiking method. It was concluded that the biosensor can be used for screening pesticide residues in food and environmental samples.
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