New turbidimetric method for determination of ciprofloxacin hydrochloride in pharmaceutical drugs using continuous flow injection manifold design with CIP.HCL-sodium nitro prusside system

Abstract

A new, simple, fast and sensitive method was developed for the determination of Ciprofloxacin. HCI pure from and drugs (tablets) by continuous flow injection scatter light. The method was based on the reaction of the Ciprofloxacin. HCI with sodium nitro prussside to form a precipitate, using homemade ISNAG-FIuorimeter. Optimum parameters have been studied to increase the sensitivity for the developed method. The scatter plot range and the linear dynamic range for the instrument response versus Ciprofloxacin. HCI concentration was 0.03-15 mmoIl L while the L.O.D was 65.3660 µg/sample from the step wise dilution for the minimum concentration of lowest concentration in the scatter plot range of the calibration graph. The correlation coefficient (r) was 0.9150 and 0.9972 while percentage linearity (R2%) was 83.73% and 99.44 for the scatter plot range and the linear dynamic range, respectively. RSD % for the repeatability (n=8) was lower than 0.2% for the determination of ciprofloxacin. HCI, with concentration of 4,13 mmol/L. The developed method was applied successfully for the determination of ciprofloxacin.HCI in pharmaceutical tablets. A comparison was made between the newly-developed method with the classical method, i.e. Turbidemtric method, of analysis using the standard addition method via the use of paired t-test. It showed that there was no significant difference between the quoted values of each individual company with calculated t-value at 95% confidence interval from developed method.