Abstract
A specific, sensitive and simple method was used for the determination of: vitamin B9 (Folic acid) in pure and pharmaceutical formulations using continuous flow injection analysis. The method is based on formation of ion pair compound between folic acid and ammonium molybdate in an aqueous medium to obtain a gray precipitate complex, using homemade; Ayah-6SX1-ST-2D solar cell CFI Analyzer. Optimum parameters was studied to increase the sensitivity for developed method. The linear range for the calibration graph was 0.01-0.6 mMol.L-1 of vitamin B9 and LOD was 131.994 ng/sample with correlation coefficient ( r ) of 0.9810, RSD% was lower than 0.1%, (n=9) for the determination of vitamin B9 at concentration (0.07and 0.5) mMol.L-1 respectively. The developed method was applied successfully for the determination of vitamin B9 in pharmaceutical tablets. A comparison was made between two methods: developed method and the classical UV spectrophotometric method at ?max=255 nm, by using the standard addition method via the use of paired t-test. It showed that there was no significant difference between the developed method and the classical method for determination vitamin B9 at 95% confidence level.
Highlights
Folate, forms of which are known as folic acid and vitamin B9, is one of the B vitamins[1]
Folic acid helps your body produce and maintain new cells, and helps prevent changes to DNA that may lead to cancer
The first line represents the carrier stream at 1.4 ml.min-1 flow rate which leads to the injection valve to carry a sample volume
Summary
Forms of which are known as folic acid and vitamin B9, is one of the B vitamins[1]. The first line represents the carrier stream (distilled water) at 1.4 ml.min-1 flow rate which leads to the injection valve to carry a sample volume (110μl of vit.B9 uses open valve mode, while the second line supplies Ammonium molybdate solution at 1.5 ml .min-1 .Both lines met at a Y-junction ,with an outlet for reactants product from complex, which passes through a homemade Ayah 6SX1-ST-2D solar cell CFI Analyzer, applied voltage to the LEDs source (six snow white light emitting diodes) was 2.08 volt DC. A series of the precipitating reagent [Am]solutions (0.005 -0.7) mMol.L-1 were prepared at constant concentration of vitamin B9 0.5mMol.L1, 110 μl sample volume at (1.4&1.5)ml.min-1flow rate for carrier stream and reagent respectively .The intensity of incident light of LEDs 2.08 V was used .Fig .4A shows the increase in concentration of Am leads to increase in the shoulder of response up to.
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