Assessment of long distance chasing photometer (NAG-ADF-300-2) by estimating the drug atenolol with ammonium molybdate via continuous flow injection analysis

Elham N Mezaal N Mezaal

doi:10.21123/bsj.2020.17.1.0078

Abstract

Atenolol was used with ammonium molybdate to prove the efficiency, reliability and repeatability of the long distance chasing photometer (NAG-ADF-300-2) using continuous flow injection analysis. The method is based on reaction between atenolol and ammonium molybdate in an aqueous medium to obtain a dark brown precipitate. Optimum parameters was studied to increase the sensitivity for developed method. A linear range for calibration graph was 0.1-3.5 mmol/L for cell A and 0.3-3.5 mmol/L for cell B, and LOD 133.1680 ng/100 µL and 532.6720 ng/100 µL for cell A and cell B respectively with correlation coefficient (r) 0.9910 for cell A and 0.9901 for cell B, RSD% was lower than 1%, (n=8) for the determination of atenolol at concentration (0.5, 0.7 and 5) mmol/L respectively. The results were compared with classical method UV-Spectrophotometric at λ max=270 nm using the standard addition method via the use of t-test, at 95% confidence level. The comparison of data explain that long distance chasing photometer (NAG-ADF-300-2) is the choice with excellent extended detection and wide application.

Highlights

Atenolol chemically known as Benzeneacetamide, 4-[2-hydroxy-3-[(1
The role of atenolol in the treatment of high blood pressure is less favorable than propranolol, as the usual dose carries the risk of diabetes [4,5].Several methods depend on continuous flow injection analysis [6,7,8,9,10,11,12,13,14,15,16,17] and several analytical methods have been reported for the determination of atenolol such as high performance liquid chromatographic(HPLC) (1822), Uv-Vis spectrophotometry [4, 23,24,25,26,27], chromatographic densitometry [3], reflectance spectroscopy [28], spectrofluorometry method [29], potentiometric titration [30],GC-Mas [5]
The first flow cell is of 110 mm length covered with 11 white snow LED (WSLED) followed by uncovered distance of 100 mm length attached to another with 2 solar cell at each side of (0-1800and 0-900 ) cell which is covered by 6 WSLED and a single photo cell of 60 mm length at each side was used with peristaltic pump (Ismatec, Switzerland) and six-port medium pressure injection valve (IDEX Corporation, USA) with sample loop (1 mm i.e. Teflon, variable length)

Summary

Introduction

Atenolol chemically known as Benzeneacetamide, 4-[2-hydroxy-3-[(1-methylethyl) amino] proxy] or called Tenormin.White powder, Molecular Formula C14 H22 N2O3, Molecular Weight 266.336 g/mol. Stability of atenolol in acidic environment depending on diversified polarity, structure of atenolol Fig. 1 [3]. Sciences (Ibn-Al-Haitham), University of Baghdad, Atenolol is classified as selective β1-receptor and is sometimes written β-blockers. The role of atenolol in the treatment of high blood pressure is less favorable than propranolol, as the usual dose carries the risk of diabetes [4,5].Several methods depend on continuous flow injection analysis [6,7,8,9,10,11,12,13,14,15,16,17] and several analytical methods have been reported for the determination of atenolol such as high performance liquid chromatographic(HPLC) (1822), Uv-Vis spectrophotometry [4, 23,24,25,26,27], chromatographic densitometry [3], reflectance spectroscopy [28], spectrofluorometry method [29], potentiometric titration [30],GC-Mas [5]

Methods

Results

Conclusion