New Turbidimetric Method for Determination of Cefotaxime Sodium in Pharmaceutical Drugs Using Continuous Flow Injection Manifold Design with CFTS- Vanadium Oxide Sulfate System

Abstract

The determination of Cefotaxime sodium pure from and pharmaceuticals (tablets) by continuous flow injection scatter light has been created using a new, simple, rapid, and sensitive approach. Using a handmade ISNAG-FIuorimeter, the technique was predicated on the interaction of Cefotaxime sodium with vanadium oxide sulfate to produce a precipitate. The optimal value had a linear dynamic range of 0.01-20 mmoI/L for the instrument response to Cefotaxime sodium concentration, while the L.O.D was 0.334 g /sample from the stepwise dilution for the lowest concentration in the calibration graph's scatter plot range. For the scatter plot range and the linear dynamic range, the correlation coefficient (r) was 0.9769 and 0.9978, respectively, while percentage linearity (R2% percent) was 95.43 percent and 99.56 percent, respectively. With a concentration of 2.5 mmol/L, the RSD percent for repeatability (n=8) was lower than 0.23% percent for the determination of cefotaxime sodium. The proposed method was effectively used to determine the concentration of cefotaxime sodium in pharmaceutical tablets. The newly developed technique was compared with the conventional methods of analysis, i.e., Turbidemtric method and UV-spectrophotometer method, utilizing the usual addition method and paired t-test. The computed t-value at 95 percent confidence interval from the established approach revealed that there was no significant difference between the quoted figures of any individual firm.