Abstract
Functionalized copolymers were synthesized and are proposed as hosts of RNA. The copolymers are based on carboxymethyl cellulose and poly-(ethylene glycol)-OH. These copolymers were functionalized with two amino acids, either lysine or histidine, through amide bond formation. The functionalized copolymer was then used to adsorb ribosomal RNA. The RNA loading was based on the nature of the amino acid functionalization of the copolymer. The array of RNA-copolymers was observed to be soft sphere-like, where the density of spheres was a function of the molecular weight of the carboxymethyl cellulose and the nature of the amino acid. Such RNA-copolymer systems are very sensitive to changes in pH.
Highlights
Nanoparticles are already being developed as effective carriers of drugs and gene delivery to target regions of the body that were previously hard to access using traditional drug formulation methods
The spectra of copolymers 3CMC-polyethylene glycol (PEG) and 7CMC-PEG fit relatively well with that of celluloses; it must be emphasized that the bands at 1740 cm−1 for 3CMC-PEG and at 1742 cm−1 for 7CMC-PEG are ascribed to the C=O stretching mode of the ester groups [30, 31]
PEGylated link polymers of carboxymethyl cellulose were effectively synthesized by EDC-NHS reaction and coupling amino acids onto the side groups of CMCPEG copolymers
Summary
Nanoparticles are already being developed as effective carriers of drugs and gene delivery to target regions of the body that were previously hard to access using traditional drug formulation methods. Functionalization of copolymers with amino acids Water at pH 5 was used to dissolve 7CMC-PEG or 3CMC-PEG. In both amino acid-free copolymers, the particle size increases approximately 3 nm after RNA incorporation.
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