New approaches to laboratory diagnosis of liver condition in cattle

Abstract

Relevance . Today the scientific community all around the world is showing significant interest to the study of endogenous intoxication syndrome in the body. The article presents the results of assessing the level of endogenous intoxication in diseases of the liver of cattle by the concentration of molecules of medium mass in the blood serum. Methods . Two groups of cows were formed, 50 animals each: 1 — healthy livestock; 2 — with liver pathology. The state of the liver in cows was assessed by the clinical status, the boundaries of the liver and its sensitivity, biochemical blood tests and ultrasound diagnostics of the hepatobiliary system. The level of endogenous intoxication in cows was studied using two MMM fractions at wavelengths = 254 nm (MMM 254) and = 280 nm (MMM 280). Results . The highest values of markers of endotoxicosis in the blood were observed in cows with hepatitis. Thus, a significant difference in MMM 254 (toxic fraction) with healthy animals in this subgroup was 1.8 times, changes in the MMM 280 fraction (fraction containing aromatic amino acids) were less pronounced — there wasan increase of 25.4%. The level of MMM 280 in the blood of cows with hepatosis did not significantly change in relation to the data of healthy livestock, at the trend level, an increase of 10.1% was observed. The level of MMM 254 at the same time significantly increased by 1.5 times. The calculation of IR 280/254 in cows with hepatopathology revealed a decrease in the index with maximum changes in animals with hepatitis; relative to healthy livestock, there was a difference of 30.4% (hepatitis) and 25.3% (hepatosis).