Abstract
A new and simple procedure for the spectrophotometric determination of the tricyclic antidepressant drug amitriptyline is proposed. The method is based on enhancement of sensitivity of the [AMIYTP]+ β-cyclodextrin and PEG molecules involved in formation of molecules inclusion complex, in presence of polyethylene glycol (PEG) medium. The mole ratio of [AMIYTP]+ β-cyclodextrin and PEG molecules in inclusion complex were determined by the curve fitting method. The value of molar absorptivity of {[AMIYTP: (β CD)] PEG} complex in term of the drug lies in rage of (2.20 - 2.23) × 104 L·mole–1·cm–1 at absorption maximum 242 nm. The slope, intercept and correlation co-efficient were found to be 14.21, 0.0046, and 0.998, respectively. The effect of analytical variables on the determination of the drug and composition of the ion associated complex are discussed in the paper. This method is applicable in the determina-tion of amitriptyline in the pharmaceutical preparations.
Highlights
Amitriptyline [3-(10,11-dihydro-5H-dibenzol[a,d]cyclohept-5-ylidene) propyldimethylamine] constitute an important class of neurotherapeutics [1] belonging to the first generation of antidepressant drug [2,3] The former has a carbocyclic structure with an exocyclic double bond at C-5 which is substituted with an N, N-dimethyl1-propanamino side chain (Figure 1)
The method is based on enhancement of sensitivity of the [AMIYTP]+ β-cyclodextrin and PEG molecules involved in formation of molecules inclusion complex, in presence of polyethylene glycol (PEG) medium
Amitriptyline gives ion-associate species with β-CD and PEG system and the absorbance increases with increase in the concentration of amitriptyline
Summary
Amitriptyline [3-(10,11-dihydro-5H-dibenzol[a,d]cyclohept-5-ylidene) propyldimethylamine] constitute an important class of neurotherapeutics [1] belonging to the first generation of antidepressant drug [2,3] The former has a carbocyclic structure with an exocyclic double bond at C-5 which is substituted with an N, N-dimethyl1-propanamino side chain (Figure 1). It is believed that amitriptyline acts by blocking the receptors of neurotransmitters, naradrenaline in the synopsis in the central nervous system, which results an increase of concentration of both molecules with a subsequent—enhancement of their antidepressant patency [3] This drug suffers from several drawbacks, such as antiarrhytmic, anticholinergic, cardiovascular and/or hyperthermia side effect [2,3], which may be reduced if the drugs are suitably vectored to the organism. Various analytical methods have been reported for determination of amitriptyline including spectrofluorometric [8], fluorospectrophotometric [9,10,11,12,13,14] flow injection method [15] atomic absorption spectroscopic [16], conductometric [17,18], high performance liquid chromatographic [19,20], voltammetric [21] and chromatographic [22] methods Some of these methods are not Figure 1. This method has been applied for determination of amitriptyline in pharmaceutical preparations
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