Abstract
The paper describes a new and simple approach for spectrophotometric determination of tricyclic antidepressant drug amitriptyline. Enhancement of the colour intensity of the Fe(III)-SCN−complex on addition of the drug amitriptyline forms the basis of the proposed method. The value of molar absorptivity of the Fe(III)-SCN−amitriptyline ion pair complex in terms of the drug lies in the range of (2.82–3.36) × 103 L·mol−1·cm−1at the absorption maximum 460 nm. The detection limit of the method was 0.3 μg·mL−1. The slope, intercept, and correlation coefficients for the present method were found to be 0.008, 0.002, and +0.998, respectively. The effect of analytical variables on the determination of the drug and the composition of the complex are discussed in the paper. The method is applicable in the determination of amitriptyline in pharmaceutical preparations.
Highlights
Amitriptyline [3-(10,11-dihydro-5H-dibenzol[a,d]cyclohept5-ylidene) propyldimethylamine] constitutes an important class among the neurotherapeutics belonging to first generation of antidepressant drug [1, 2]
It is believed that drug amitriptyline acts by blocking the receptors of neurotransmitters, noradrenalin, in the synopsis in the central nervous system, which results in an increase of concentration of both molecules with a subsequent enhancement of their antidepressant potency
Various analytical methods have been reported for determination of amitriptyline including spectrofluorimetric [9], spectrophotometric [10,11,12,13,14,15], flow injection method [16], UV-Visible spectrophotometric methods [17, 18] based on chromogenic reaction with citric acid-acetic anhydride [19], bromothymol blue [20], ammonium reineckate [21, 22], bismuth-hexaiodide [23], and cerium (IV) [24]
Summary
Amitriptyline [3-(10,11-dihydro-5H-dibenzol[a,d]cyclohept5-ylidene) propyldimethylamine] constitutes an important class among the neurotherapeutics belonging to first generation of antidepressant drug [1, 2]. Various analytical methods have been reported for determination of amitriptyline including spectrofluorimetric [9], spectrophotometric [10,11,12,13,14,15], flow injection method [16], UV-Visible spectrophotometric methods [17, 18] based on chromogenic reaction with citric acid-acetic anhydride [19], bromothymol blue [20], ammonium reineckate [21, 22], bismuth-hexaiodide [23], and cerium (IV) [24] Most of these methods suffer from several interferences by many basic compounds, until several manipulation steps, and can be applied only to relatively high concentration of drugs. This method has been applied for determination of the concentration of amitriptyline in pharmaceutical preparations
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