Abstract
Considering the undesirable attributes of synthetic fungicides and the availability of Ficus species in China, the stem of Ficus tikoua Bur. was investigated. One new antifungal pyranoisoflavone, 5,3′,4′-trihydroxy-2″,2″-dimethylpyrano (5″,6″:7,8) isoflavone (1), together with two known isoflavones, wighteone (2) and lupiwighteone (3) (with previously reported antifungal activities), were isolated from ethyl acetate extract by bioassay-guided fractionation. Their structures were determined by spectroscopic analysis, such as NMR (1H-1H COSY, HMQC, HMBC and NOESY), IR, UV and HRMS, as well as ESI-MSn analyses. The antifungal activities of 1–3 against Phytophthora infestans were evaluated by direct spore germination assay, and the IC50 values were 262.442, 198.153 and 90.365 μg·mL−1, respectively.
Highlights
The plants of the Ficus genus have attracted considerable attention from pharmacologists due to a wide range of biological properties
Previous studies show that the extracts of the plant exhibit excellent antifungal activity against many species of pathogens, such as Pseudoperonospora cubensis, Phytophthora infestans and
That the 2,2-dimethylchromen residue is at the 7,8-position in 1 was established based on the analysis of heteronuclear multiple-bond correlation (HMBC) spectrum and by comparing the spectral data of its monomethyl ether with that reported in literature [10,11]
Summary
The plants of the Ficus genus have attracted considerable attention from pharmacologists due to a wide range of biological properties. Ficus tikoua Bur., a wood plant of the Ficus genus, is widely distributed in southern China, India, Vietnam and Laos. It has long been used in traditional folk medicine to treat diseases, such as chronic bronchitis, diarrhea, dysentery, rheumatism, edema, impetigo, and so on [1,2,3]. Previous studies show that the extracts of the plant exhibit excellent antifungal activity against many species of pathogens, such as Pseudoperonospora cubensis, Phytophthora infestans and. The plant can be considered as a potential botanical pesticide. We report the isolation, structural elucidation and antifungal activities of a new pyranoisoflavone, named 5,3',4'-trihydroxy-2",2"-dimethylpyrano (5",6":7,8) isoflavone (1), along with other two known isoflavones, wighteone (2) and lupiwighteone (3), whose antifungal activities were reported previously [5] (Figure 1)
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