Abstract
AbstractAn avirulent strain of Pseudomonas solanacearum could inhibit the growth of its virulent parent on L‐tryptophan‐containing glycerol nutrient agar (TGNA) medium. It was, also, capable of inhibiting, though to a less degree, Corynebacterium fascians and Pseudomonas marginata, out of five other bacterial species tested. While P. marginata was partially inhibited by the avirulent strain it was totally insensitive to indole‐3‐acetic acid (IAA) up to a concentration of 300 μg/ml. Additionally, Erwinia carotovora var. atroseptica which was totally unaffected by the avirulent strain showed a spectrum of sensitivity to IAA concentrations close to that of the virulent strain. No DNA, RNA, or IAA could ever be detected in the inhibition area and, thus, it is almost certain that the inhibiting agent produced by the avirulent strain is not IAA as was previously speculated. This inhibiting agent was insensitive to autoclaving and to the enzymes, pronase, trypsin, DNAse, and RNAse. P. solanacearum bacteriocin was detected by polyacrylamide gel electrophoresis in the medium near the avirulent growth line and not throughout the inhibition area. This supports the conclusion that bacteriocin alone cannot be held responsible for the inhibition phenomenon observed and leaves the nature of this inhibiting agent unknown.
Published Version
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