Negative frequency-dependent growth underlies the stable coexistence of two cosmopolitan aquatic plants.

Abstract

Identifying and quantifying the mechanisms influencing species coexistence remains a major challenge for the study of community ecology. These mechanisms, which stem from species' differential responses to competition and their environments, promote coexistence if they give each species a growth advantage when rare. Yet despite the widespread assumption that co-occurring species stably coexist, there have been few empirical demonstrations in support of this claim. Likewise, coexistence is often assumed to result from interspecific differences in life-history traits, but the relative contributions of these trait differences to coexistence are rarely quantified, particularly across environmental gradients. Using two widely co-occurring and ecologically similar species of freshwater duckweed plants (Spirodela polyrhiza and Lemna minor), we tested hypotheses that interspecific differences in facultative dormancy behaviors, thermal reaction norms, and density-dependent growth promote coexistence between these species, and that their relative influences on coexistence change as average temperatures and fluctuations around them vary. In competition experiments, we found strong evidence for negative frequency-dependent growth across a range of both static and fluctuating temperatures, suggesting a critical role of fluctuation-independent stabilization in coexistence. This negative frequency dependence could be explained by our observation that for both species, intraspecific competition was over 1.5 times stronger than interspecific competition, granting each species a low-density growth advantage. Using an empirically parameterized competition model, we found that while coexistence was facilitated by environmental fluctuations, fluctuation-independent stabilization via negative frequency dependence was crucial for coexistence. Conversely, the temporal storage effect, an important fluctuation-dependent mechanism, was relatively weak in comparison. Contrary to expectations, differences in the species' thermal reaction norms and dormancy behaviors did not significantly promote coexistence in fluctuating environments. Our results highlight how coexistence in two ubiquitous and ostensibly similar aquatic plants is not necessarily a product of their most obvious interspecific differences, and instead results from subtle niche differences causing negative frequency-dependent growth, which acts consistently on both species across environmental gradients.