Negative effects of long-term moderate salinity and short-term drought stress on the photosynthetic performance of Hybrid Pennisetum

Abstract

Plants are always suffering periods of soil water deficit and sustained soil salinity during their life cycle. Unraveling the mechanisms underpinning the responses of plants, especially the photosynthesis, to drought, salinity, and co-occurring stresses is critical for both the protection of natural vegetation and the stabilization of crop production. To better understand the downregulation of photosynthetic capability induced by soil salinity and drought, gas exchange parameters, leaf pigment contents, and chlorophyll (Chl) a fluorescence transients were analyzed in leaves of Hybrid Pennisetum. Our results showed that long-term moderate salinity, short-term drought, and the combination of these stressors decreased leaf pigment content by 11.4–31.5% and net photosynthetic rate (Pn) by 14.6–67.6% compared to those in untreated plants. The reduction of Pn in Hybrid Pennisetum under long-term salinity stress mainly occurred by stomatal limitation, whereas non-stomatal limitation played a dominant role under short-term drought stress. The changes in Chl a fluorescence kinetics (especially the appearance of the L-band and K-band) in both stress treatments showed that salinity and drought stress damaged the structural stability of photosystem II (PSII) and disturbed the equilibrium between the electrons at the acceptor and donor sides of PSII. Furthermore, although the negative effect of drought stress on leaf photosynthesis was much greater than that of salinity stress, moderate salt stress alleviated the negative effect of drought stress on the photosynthetic performance of Hybrid Pennisetum after long acclimation times.