Natural v/s Commercial trypsin isolated from liver waste of Labeo rohita

Abstract

The mounting human population and the massive amount of waste generated from the same is receiving particular attention towards valorisation of waste. According to the annual report of FAO, the human consumption of fish protein has reached 87% in 2016 from 67% during 1960s [1]. Aquaculture only has contributed to 5.8% annual growth rate among food sectors in the past decade. In this milieu, disposal of fish visceral waste is becoming a major menace to fishery industries exerting a great economic and environmental impact. Being perishable in nature, the organic portion of the waste decomposes rapidly and acts as a breeding ground for microbes. Moreover, the hefty and indiscriminate use of antibiotics and disinfectants in farmed animals is developing resistant strains, thus raising environment and ecological concerns. In order to solve such problem, the present investigation focussed upon employing the visceral trypsin as a cell dissociating agent. The efficacy of trypsin obtained from viscera of Labeo rohita upon KB cell line (Doubling time 50 hrs.) was assessed in terms of cell viability. The cytotoxic effect of the visceral trypsin at 0.01%, 0.1% and 1% concentration were investigated at three time points (10 sec, 15 sec and 20 sec). Commercial (bovine) trypsin was considered as control. A time dependent decrease in cell viability upon gradually increasing the concentration was observed in all groups of treatment. The lowest reduction in cell viability (2%) was observed with 1% concentration at 15 sec and 20sec. Although, commercial trypsin was found more efficient than trypsin isolated from waste during this study but the potency of visceral trypsin observed cannot be ruled out. Thus, the application of this enzyme as a cell-dissociating agent suggested it as a comparable candidate with commercial trypsin.