Natural Formulations Provide Antioxidant Complement to Hyaluronic Acid-Based Topical Applications Used in Wound Healing.

Pooyan Makvandi,Rosanna Veneziano,Stefania Zeppetelli,Assunta Borzacchiello,Francesca Della Sala,Caterina Caccavale

doi:10.3390/polym12081847

Abstract

Hyaluronic acid (HA) promotes wound healing, and, accordingly, formulations based on HA have been widely used in regenerative medicine. In addition, naturally derived compounds, e.g., plant-based extracts and vitamin E, have exhibited antioxidant activity. In this study, a formulation containing hyaluronic acid, vitamin E, raspberry extract, and green tea was developed for potential topical applications, targeting wound healing. Rheological analysis was performed along with antioxidant and biological studies. The rheological characterization showed that the HA-based formulation is a thixotropic platform and possesses higher mechanical properties than the control formulation. To evaluate the wound healing potential of the formulation, an in vitro “wound healing” assay was carried out using human derived fibroblasts (HDF) with a cell-free gap on the tissue culture dish. The formulation showed better wound healing ability than the control formulation.

Highlights

Wound dressings can promote the healing process and reduce the healing time [1,2]
The results indicate that the HA-based natural formulation (HBN) formulation possesses antioxidant activity while the control formulation does not have this capability
Hyaluronic acid (HA)-based formulations were enriched with ingredients exhibiting antioxidant activity including vitamin E and natural compounds, such as raspberry and green tea, to create a formulation to promote the healing process in topical applications

Summary

Introduction

Hyaluronic acid (HA) is naturally occurring polysaccharide widely employed in regenerative medicine and in particular for topical and intradermal applications. Hyaluronic acid promotes wound healing, and, formulations containing HA have been applied in regenerative medicine. Natural polysaccharides such as HA have been employed as drug carriers for biomedical applications along with many pharmaceutical formulations to enhance the biocompatibility of the final bioproduct [3,4,5]. Antioxidant agents exhibit encouraging results in the wound healing process [6]. Radicals such as reactive oxygen species (ROS) play an important role in healing processes and in carcinogenesis. At least, diminish ROS production, which occurs during skin aging and cancer, attaining antioxidants from several sources, e.g., food supplements and skincare products, Polymers 2020, 12, 1847; doi:10.3390/polym12081847 www.mdpi.com/journal/polymers

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