Abstract
The present study aimed to develop nanoliposomes codelivering multiple bioactive peptides to achieve improved and comprehensive anti-aging effects in human skin. The nanoliposomes were simultaneously loaded with carnosine, palmitoyl tripeptide-5 and acetyl hexapeptide-3 (CPA-NLPs) for topical delivery. The CPA-NLPs were nano-scaled with narrow size distribution, and showed high encapsulation efficiency, high loading capacity and sustained release of bioactive peptides. Flow cytometry and confocal laser scanning microscopy showed that the nanoliposomes significantly enhanced the cellular uptake of the loaded cargo by human skin fibroblasts (HSF) cells. The CPA-NLPs also protected HSF cells from H2O2-induced oxidative stress damage and increased their expression levels of type Ι collagen and hyaluronic acid. In addition, treatment with CPA-NLPs inhibited catecholamine release in rat adrenal pheochromocytoma (PC12) cells. In a clinical trial, application of a lotion containing 2% CPA-NLPs in face led to a remarkable reduction of the volume, area and area ratio of wrinkles, and an improvement of skin elasticity. These results suggest that loading a combination of bioactive peptides into nanoliposomes is a promising strategy to enhance the topical delivery efficiency and the anti-aging efficacy.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
